Protein Purification Reagents and Kits
ProteinSimple Maurice cIEF pI Marker - 6.14, Lyophilized, 210 uL
Maurice C. reagents and consumables are designed for use on with Maurice and Maurice C. systems, and must be used in conjunction with Compass for iCE version 1.0 software.
STEMCELL Technologies EasySep™ RBC Depletion Reagent - 10mL, For processing: 100 mL whole blood processed
The EasySep™ RBC Depletion Reagent is designed to immunomagnetically deplete red blood cells (RBCs) from fresh human peripheral blood, umbilical cord blood, bone marrow or leukapheresis samples by negative selection. RBCs are targeted with Tetrameric Antibody Complexes recognizing Glycophorin A and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Unwanted cells remain in the tube while desired cells are poured off.This product replaces the EasySep™ Human Glycophorin A Depletion Kit (Catalog #18352) for even faster cell isolations.
STEMCELL Technologies EasySep™ Human Monocyte Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Monocyte Isolation Kit is designed to isolate CD14+CD16- monocytes from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells and platelets with antibody complexes and magnetic particles. The magnetically labelled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.This product can be used in place of the EasySep™ Human Monocyte Enrichment Kit (Catalog #19059) for even faster cell isolations with reduced platelet contamination.
STEMCELL Technologies EasySep™ Magnet, For isolating: 1 Unit
The EasySep™ Magnet is designed for cell separation procedures using EasySep™ reagents. The EasySep™ Magnet generates a high-gradient magnetic field in the interior cavity that is strong enough to separate cells labeled with EasySep™ Magnetic Particles without the use of columns. This magnet is designed to hold a standard 12 x 75 mm (5 mL) polystyrene tube.
Aniara Diagnostica LLC ANTI - XA
This BIOPHEN™ ANTI-Xa (2 Stages heparin Assay) kit is a two-stage chromogenic assay for measuring the activity of heparins (UFH or LMWH), in manual or automatic method. This method is proposed only to test heparin in human citrated plasma, or in purified solution. This kit is for research use only and must not be used from patient diagnosis or treatment. NOTE: This product replaces Ref #221010 and requires separate purchase of specific buffers depending on the analyte being tested and compendia reference desired: Specific buffer needed for UFH Assay, USP or EP: AR030K Specific buffers needed for LMWH Assay, USP: AR005L, AR029K, AR028K Specific buffers for LMWH Assay, EP: AR005L, AR029K
FUJIFILM BIOSCIENCES INC MAGCAPTURE EXOSOME ISOLATN KIT
MagCapture Exosome Isolation Kit PS Version 2 10 Tests
System Biosciences LLC PEG-it Virus Precipitation Solution, 100ml
PEG-it Virus Precipitation Solution (100 mL aliquot)
Takara Bio PrimeSTAR® Max DNA Polymerase, 100 Rxns
A 2X hot-start PCR master mix that is ideal for high-fildelity PCR or high-throughput applications. PrimeSTAR Max DNA polymerase pairs Takara Bio's high-fidelity PrimeSTAR HS DNA Polymerase with an elongation factor to provide efficient priming and extension, thereby shortening annealing and extension times. Please refer to the User Manual for the master mix composition. 100 Rxns
IBA LifeSciences Twin-Strep-tag Capture Kit for SPR analysis; Classic size
Twin-Strep-tag Capture Kit is intended for site-directed, reversible capture of Twin-Strep-tag fusion proteins for biomolecular interaction analysis using surface plasmon resonance (SPR) devices. The kit provides Strep-TactinXT, SPR Immobilization Buffer, Regeneration Buffer, and GFP-Twin-Strep-tag as control protein. It enables the highly stable immobilization of the ligand based on the unique Strep-TactinXT:Twin-Strep-tag interaction, which operates with pM affinity. The key benefits are:• Indirect immobilization protecting the ligand from chemical modification as well as inactivation or manipulation by the immobilization procedure• High affinity enabling measurements over a long period• Chip can be regenerated easily by an established procedure• Ligands can be captured directly from complex samples due to the high affinity and specificityThe use of unconjugated Strep-TactinXT requires a license that is provided with the purchase of the kit as limited use license.
Pfanstiehl Inc Maltose Monohydrate. High Purity, Low Endotoxin, Low Metals, USP-NF, JP, ChP (M-167) -100G GMP, Multi-compendial CAS: 6363-53-7
Pfanstiehl Maltose Monohydrate High Purity, Low Endotoxin, Low Metals is a multi-compendial carbohydrate utilized in bioprocessing applications and as an infusion stabilizer. Pfanstiehl Maltose has been specifically designed and formulated to remove impurities such as endotoxin, trace metals, and nitrosamines. Manufactured in the USA under ICH-Q7 cGMP conditions, Pfanstiehl Maltose meets parenteral (injectable) excipient grade standards, making it ideal as a bioprocessing feed, or as a stabilizing agent for pharmaceutical infusions. Pfanstiehl Maltose is available as USP-NF (National Formulary) JP (Japanese Pharmacopeia) and ChP (Chinese Pharmacopeia) Compendial, U.S.P. , cGMP , pharmaceutical grade .
IBA LifeSciences Strep-TactinXT 4Flow resin as 50% suspension; 20 ml
Strep-TactinXT 4Flow is a 4% agarose coupled with the streptavidin variant Strep-TactinXT and applicable for purification of Strep-tagII or Twin-Strep-tag fusion proteins. Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (> 95%), and highest binding affinity, the resin is recommended for all protein classes, especially for low abundant or challenging proteins. The low concentrated agarose allows for the simple attachment of larger proteins to the stationary phase, making it particularly suitable for purification of high-molecular-weight targets. In addition, Strep-TactinXT 4Flow enables the application of denaturing agents such as urea. The suspension can be used for packing of gravity flow columns since the resin is optimized for column affinity chromatography, rather than for batch purification. To pack a column with 1 ml resin bed volume, 2 ml 50% suspension are necessary.
Wl Gore And Associates 1.0 mL GORE Protein Capture Device with Protein A for Rapid mAb Purification
GORE Protein Capture Devices with immobilized Protein A are intended for small-scale affinity purification of monoclonal antibodies and other proteins demonstrating an Fc region. GORE Protein Capture Devices use a unique expanded polytetrafluoroethylene (ePTFE) membrane composite that provides a binding capacity advantage at high flow rates and improved the speed of purification. In addition the GORE Protein Capture Deivces produce highly concentrated elution pools, which may eliminate a downstream concentration step during the antibody purification process. These unique membrane devices are offered in 1 mL (≥ 30 mg capacity), 3.5 mL (≥ 105 mg capacity) and 9.0 mL (≥27 mg capacity) at 20 seconds residence time.
STEMCELL Technologies EasySep™ Release Human CD4 Positive Selection Kit, For processing: 1 x 10^9 cells
The EasySep™ Release Human CD4 Positive Selection Kit is designed to isolate CD4+ cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic positive selection.Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated CD4+ cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands. The CD4 antigen is strongly expressed on helper T cells and is expressed more weakly on peripheral blood monocytes and tissue macrophages.
Protifi LLC S-Trap mini kit (10x 100 – 300 ug)
S-Trap sample processing is now available in a full kit format. The kit includes lysis, wash, and digestion buffers. The user must supply HPLC grade MeOH.