Protein Purification Reagents and Kits
Agilent Technologies AGILENT BIONYLZR TEST CHIP KIT
Test Chip Kit. For electrophoresis instrument troubleshooting and diagnostics. Includes autofocus chip, electrode/diode test chip, and documentation.
STEMCELL Technologies EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Kit is designed to isolate highly purified CD25+ cells from single-cell suspensions of splenocytes or other tissues by positive selection. The kit targets CD25 regulatory T cells for positive selection with a PE-labeled antibody directed against the CD25 surface marker. Desired cells are labeled with antibodies and magnetic particles. The cells are separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube.
Bio Rad Laboratories BIO-SPIN DISP COLUMN
Extra thick blot paper is cotton fiber paper that is used in the assembly of a gel and membrane sandwich for electrophoretic transfer, or blotting, applications.
ProteinSimple Maurice CE-SDS PLUS Cartridge, 2/pk
Cartridges for CE-SDS PLUS application, 2 cartridges/Pk. For use with Maurice (090-000) and Maurice S. (090-001) systems.
Macherey-Nagel Protino Glutathione Agarose 4B, 10 mL
Protino Glutathione Agarose 4B (10 mL) Protino Glutathione Agarose 4B suspension for the purification of GST-tag proteins pack of 10 mL settled beaded agarose
STEMCELL Technologies RoboSep™ Human T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human T Cell Isolation Kit is designed to isolate T cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.This product can be used in place of the EasySep™ Human T Cell Enrichment Kit (Catalog #19051) for even faster cell isolations.
Gold Biotechnology Inc Nickel NTA Agarose Beads 100 mL
Nickel-NTA Agarose Resin has a binding capacity of ~50 mg/ml. In addition, it allows for purification of proteins under native or denaturing conditions. GoldBio Nickel Agarose works very well with the His-Tag Buffer Set.
Dojindo Molecular Technologies Inc Dojindo ExoSparkler Exosome Protein Labeling Kit, Red, 1 to 10 μg Protein per Sample, 5 Samples
This kit allows for exosome protein fluorescent staining and enables observation of exosome dynamics without extracellular aggregation, covering steps from fluorescence labeling to purification. It can be used for staining purified exosomal membrane or protein, allowing imaging of labeled exosomes taken up by cells.
- Enables observation of exosome dynamics without extracellular aggregation.
- Includes filtration tubes for removal of unreacted dyes.
- Optimized protocol for exosome labeling.
- Recommended protein amount per sample: 1 to 10 μg.
- Recommended particle count per sample: 10 to 100 x 108 particles.
- Detection conditions (red): Ex 561 nm/Em 570 to 640 nm.
- Recommended purification methods include ultracentrifugation, immunoprecipitation, and magnetic beads.
- Store at 0°C to 5°C, protected from moisture.
IBA LifeSciences Twin-Strep-tag Capture Kit for SPR analysis; Classic size
Twin-Strep-tag Capture Kit is intended for site-directed, reversible capture of Twin-Strep-tag fusion proteins for biomolecular interaction analysis using surface plasmon resonance (SPR) devices. The kit provides Strep-TactinXT, SPR Immobilization Buffer, Regeneration Buffer, and GFP-Twin-Strep-tag as control protein. It enables the highly stable immobilization of the ligand based on the unique Strep-TactinXT:Twin-Strep-tag interaction, which operates with pM affinity. The key benefits are:• Indirect immobilization protecting the ligand from chemical modification as well as inactivation or manipulation by the immobilization procedure• High affinity enabling measurements over a long period• Chip can be regenerated easily by an established procedure• Ligands can be captured directly from complex samples due to the high affinity and specificityThe use of unconjugated Strep-TactinXT requires a license that is provided with the purchase of the kit as limited use license.
STEMCELL Technologies EasySep™ Direct Human T Cell Isolation Kit, For processing: 100 mL whole blood processed
This cell separation kit can be used to isolate highly purified T cells directly from human whole blood by immunomagnetic negative selection.This kit targets non-T cells for removal with antibodies recognizing specific surface markers. Unwanted cells are labeled with antibodies and EasySep™ Direct RapidSpheres™, and separated using an EasySep™ magnet. Desired cells are simply collected into a new tube and can directly be used for functional assays, flow cytometry or DNA/RNA extraction.
STEMCELL Technologies EasySep™ Human Monocyte Enrichment Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Monocyte Enrichment Kit is designed to isolate monocytes from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-monocyte cells and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to prevent nonspecific binding of monocytes. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. For applications in which CD16+ cells are not removed, we recommend the EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion (Catalog #19058). For even faster cell isolations, we recommend the new EasySep™ Human Monocyte Isolation Kit (19359) (http://www.stemcell.com/en/Products/All-Products/EasySep-Human-Monocyte-Isolation-Kit.aspx) which isolates cells in as little as 12.5 minutes.
STEMCELL Technologies EasySep™ Direct Human Total Lymphocyte Isolation Kit, For processing: 100 mL whole blood processed
The EasySep™ Direct Human Total Lymphocyte Isolation Kit isolates highly purified lymphocytes directly from human whole blood by immunomagnetic negative selection. Isolated cells are untouched and available for direct use in flow cytometry, culture, DNA/RNA extraction or other downstream applications.
Biotage GEL FILTRATION PHYTIP COLUMNS
Gel filtration PhyTip® columns (1000 µL volume) containing 600 µL of 5K size exclusion resin per column
Invent Biotechnologies Inc Minute™ Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes, 20 preps, spin-column-based, detergent-free extraction, high yield 2-3 mg/ml, 50-80 mg adipose tissue
Minute™ Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes, 20 preps, spin-column-based, detergent-free extraction, high yield 2-3 mg/ml, 50-80 mg adipose tissue